What does DNase I primarily target for digestion?

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Multiple Choice

What does DNase I primarily target for digestion?

Explanation:
DNase I is a nonspecific endonuclease that cleaves DNA by hydrolyzing phosphodiester bonds, and it works best on double-stranded DNA. It binds to the duplex and introduces nicks in the minor groove, cutting the two strands of the double helix. It can act on single-stranded DNA, but much less efficiently, and it doesn’t have specificity for particular bases or RNA. So the primary substrate it digests is double-stranded DNA.

DNase I is a nonspecific endonuclease that cleaves DNA by hydrolyzing phosphodiester bonds, and it works best on double-stranded DNA. It binds to the duplex and introduces nicks in the minor groove, cutting the two strands of the double helix. It can act on single-stranded DNA, but much less efficiently, and it doesn’t have specificity for particular bases or RNA. So the primary substrate it digests is double-stranded DNA.

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